Kewal Asosingh, PhD, SCYM(ASCP)
Associate Staff
Board Certified Specialist in Cytometry
Email: [email protected]
Location: Cleveland Clinic Main Campus
Research
Our laboratory specializes in conducting preclinical testing using experimental models of asthma and pulmonary arterial hypertension. Additionally, we engage in collaborative research to investigate the role of abnormal blood vessels and bone marrow stem cells in the development and progression of lung diseases. Our team focuses on providing skilled biotechnological services, such as 4-dimensional functional lung imaging, measurements of airway hyperresponsiveness, assessment of right ventricle hemodynamics, and high-dimensional spectral flow cytometric immunophenotyping of inflammatory and lung-resident cells.
Biography
Dr. Kewal Asosingh is an Associate Staff in the Department of Inflammation and Immunity and in the Department of Cores Administration. He specializes in preclinical asthma and pulmonary arterial hypertension models, as well as high-dimensional flow cytometry. As such, Dr. Asosingh is in charge of a Preclinical Lung Physiology Shared Resource, which houses a 4DMedical Preclinical imager and other pulmonary research equipment. He is also the Scientific Director of Flow Cytometry at the Lerner. His research laboratory studies myeloid mechanisms in pulmonary vascular disease and regional ventilation heterogeneity in asthma.
Education & Professional Highlights
Research Scholar, Lung Vascular Biology, Cleveland Clinic Lerner Research Institute, OH, 2004-2006 Postdoctoral Fellow, Vrije Universiteit Brussel, Brussels, Belgium, 2001-2004 Doctorate in Medical Sciences, Vrije Universiteit Brussel, Brussels, Belgium, 1997-2001 Masters in Medical and Pharmaceutical Research (Cum Laude), Vrije Universiteit Brussel, Brussels, Belgium, 1997-2001. Bachelors in Biomedical Sciences (CumLaude), Vrije Universiteit Brussel, Brussels, Belgium, 1991-1995.
"CIMER Trained Mentor" indicates the principal investigator has completed mentorship training based on curriculum from the Center for the Improvement of Mentored Experiences in Research, aimed at advancing mentoring relationships and promoting cultural change in research.
Research
- Overall areas of interest
- Airway inflammation, lung vascular biology, regional airflow
- Tools and expertise of the lab (with references)
- Endothelial Colony-Forming Cell (ECFC) assays (Duong Pulm Circ 2011, Asosingh Lung Stem Cells 2015)
- Bone marrow-derived proangiogenic hematopoietic progenitor assays (Asosingh JCI 2018 JI 2016 JACI 2010)
- Asthma models and airway hyperreactivity quantification (Asosingh JCI 2018, Ghosh PNAS 2016, Kim Immunity 2020)
- 4Dx regional airflow imaging of lung function (Thurgood J Synchrotron Radiat 2016, Murrie Sci Rep 2020)
- Pulmonary Arterial Hypertension models and Right ventricular hemodynamic measurements (Asosingh Blood Adv. 2017 Blood 2012)
- Mitochondria tracing models (Stephens Mitochondrion 2020)
- Flow Cytometry and Single-Cell Omics (Reichard Cytometry A. 2019 Cytometry A. 2018, Saygin Cytometry A. 2018)
- Examples of collaborators inside and outside of I&I
- Xioaxio Li, Dennis Stuehr, Serpil Erzurum/ Asthma Inflammation Research
- Serpil Erzurum/Nitric Oxide in Pulmonary Hypertension
- Arnab Ghosh/ sGC Dysfunction in Asthma
- Raed Dweik/ Mechanisms of Metabolic Dysregulation in Pulmonary Hypertension
- Lab discoveries
- Discovered vascular mechanisms in allergic airway inflammation and remodeling in asthma (Asosingh JCI Insight. 2020 JCI 2018 J Immunol. 2016 J Immunol. 2013)
- Discovered bone marrow contribution to the development of vascular disease in PAH (Asosingh Blood Adv. 2017 Blood 2012 Am J Pathol 2008, Farha Blood 2011, Duong Pulm Circ. 2011)
- Developed various methods and best practices in Flow Cytometry (Wanner Methods Mol Biol 2019, Reichard Cytometry A. 2019 Cytometry A. 2018, Saygin Cytometry A. 2018)
- How could these discoveries help patients?
- New therapies for asthma and pulmonary arterial hypertension
- Better understanding of disease mechanisms.
Our Team
Selected Publications
1: Rose JA, Wanner N, Cheong HI, Queisser K, Barrett P, Park M, Hite C, Naga
Prasad SV, Erzurum S, Asosingh K. Flow Cytometric Quantification of Peripheral
Blood Cell β-Adrenergic Receptor Density and Urinary Endothelial Cell-Derived
Microparticles in Pulmonary Arterial Hypertension. PLoS One. 2016 Jun
7;11(6):e0156940. doi: 10.1371/journal.pone.0156940. eCollection 2016. PubMed
PMID: 27270458; PubMed Central PMCID: PMC4896479.
2: Xu W, Ghosh S, Comhair SA, Asosingh K, Janocha AJ, Mavrakis DA, Bennett CD,
Gruca LL, Graham BB, Queisser KA, Kao CC, Wedes SH, Petrich JM, Tuder RM, Kalhan
SC, Erzurum SC. Increased mitochondrial arginine metabolism supports
bioenergetics in asthma. J Clin Invest. 2016 May 23. pii: 82925. doi:
10.1172/JCI82925. [Epub ahead of print] PubMed PMID: 27214549.
3: Ghosh A, Koziol-White CJ, Asosingh K, Cheng G, Ruple L, Groneberg D, Friebe A,
Comhair SA, Stasch JP, Panettieri RA Jr, Aronica MA, Erzurum SC, Stuehr DJ.
Soluble guanylate cyclase as an alternative target for bronchodilator therapy in
asthma. Proc Natl Acad Sci U S A. 2016 Apr 26;113(17):E2355-62. doi:
10.1073/pnas.1524398113. Epub 2016 Apr 11. PubMed PMID: 27071111; PubMed Central
PMCID: PMC4855555.
4: Asosingh K, Vasanji A, Tipton A, Queisser K, Wanner N, Janocha A, Grandon D,
Anand-Apte B, Rothenberg ME, Dweik R, Erzurum SC. Eotaxin-Rich Proangiogenic
Hematopoietic Progenitor Cells and CCR3+ Endothelium in the Atopic Asthmatic
Response. J Immunol. 2016 Mar 1;196(5):2377-87. doi: 10.4049/jimmunol.1500770.
Epub 2016 Jan 25. PubMed PMID: 26810221; PubMed Central PMCID: PMC4761512.
5: Gupta MK, Asosingh K, Aronica M, Comhair S, Cao G, Erzurum S, Panettieri RA
Jr, Naga Prasad SV. Defective Resensitization in Human Airway Smooth Muscle Cells
Evokes β-Adrenergic Receptor Dysfunction in Severe Asthma. PLoS One. 2015 May
29;10(5):e0125803. doi: 10.1371/journal.pone.0125803. eCollection 2015. PubMed
PMID: 26023787; PubMed Central PMCID: PMC4449172.
6: Barnes JW, Tian L, Heresi GA, Farver CF, Asosingh K, Comhair SA, Aulak KS,
Dweik RA. O-linked β-N-acetylglucosamine transferase directs cell proliferation
in idiopathic pulmonary arterial hypertension. Circulation. 2015 Apr
7;131(14):1260-8. doi: 10.1161/CIRCULATIONAHA.114.013878. Epub 2015 Feb 6. PubMed
PMID: 25663381; PubMed Central PMCID: PMC4390469.
7: Roach EC, Park MM, Tang WH, Thomas JD, Asosingh K, Kalaycio M, Erzurum SC,
Farha S. Impaired right ventricular-pulmonary vascular function in
myeloproliferative neoplasms. J Heart Lung Transplant. 2015 Mar;34(3):390-4. doi:
10.1016/j.healun.2014.09.009. Epub 2014 Sep 28. PubMed PMID: 25638299; PubMed
Central PMCID: PMC4377111.
8: Farha S, Dweik R, Rahaghi F, Benza R, Hassoun P, Frantz R, Torres F, Quinn DA,
Comhair S, Erzurum S, Asosingh K. Imatinib in pulmonary arterial hypertension:
c-Kit inhibition. Pulm Circ. 2014 Sep;4(3):452-5. doi: 10.1086/677359. PubMed
PMID: 25621158; PubMed Central PMCID: PMC4278604.
9: Rose JA, Erzurum S, Asosingh K. Biology and flow cytometry of proangiogenic
hematopoietic progenitors cells. Cytometry A. 2015 Jan;87(1):5-19. doi:
10.1002/cyto.a.22596. Epub 2014 Nov 21. Review. PubMed PMID: 25418030; PubMed
Central PMCID: PMC4356492.
10: Lanzola E, Farha S, Erzurum SC, Asosingh K. Bone marrow-derived vascular
modulatory cells in pulmonary arterial hypertension. Pulm Circ. 2013
Dec;3(4):781-91. doi: 10.1086/674769. Review. PubMed PMID: 25006394; PubMed
Central PMCID: PMC4070843.
11: Ratanamaneechat S, Neumann DR, Difilippo FP, Comhair SA, Asosingh K, Ghosh S,
Grandon D, Khan A, Aronica MA, Erzurum SC. Redox imaging of inflammation in
asthma. Am J Respir Crit Care Med. 2014 Mar 15;189(6):743-6. doi:
10.1164/rccm.201310-1872LE. PubMed PMID: 24628314; PubMed Central PMCID:
PMC3983839.
Careers
Research News
Renowned lung disease researcher Dr. Kewal Asosingh has established the Cleveland Clinic Flow Cytometry program as a world class institution, setting internationally recognized standards.
Researchers studied how the vascular endothelium contributes to the onset of airway inflammation, a new discovery and break from research over the past 50 years that focuses on epithelium only.
Funding was awarded from the National Institutes of Health (NIH) to purchase a leading-edge preclinical scanner from 4Dx Limited, a Melbourne-based medical technology company.