Vincent Hascall Laboratory

Research

Researchers in my laboratory work to determine the mechanisms for the aberrant synthesis of hyaluronan, a very large, negatively charged polysaccharide, in intracellular compartments when dividing cells are exposed to high glucose levels characteristic of uncontrolled diabetes. The mechanism was identified in kidney glomerular mesangial cells, and shows that cells entering division stop glucose uptake normally but cannot do so in high glucose levels. Intracellular hyaluronan in dividing cells has now been identified in hyperglycemic diabetic pathologies, and also in the current  obesity  epidemic brought on by the long-time adherence to the Western diet (low fat/high sugar).

We have also shown that low concentrations of heparin block glucose uptake within one hour in cells entering division in high glucose, and the cells sustain normal functions after division. Our ongoing research has shown that this mechanism is central to monocyte/macrophage pathologies. Monocyte progenitor cells dividing in high glucose produce circulating monocytes that contain intracellular hyaluronan, have a pro-inflammatory phenotype, and cannot successfully clear pathological hyaluronan matrices formed during inflammations. The presence of heparin prevents the intracellular hyaluronan response and allows the circulating monocyte to become a functional tissue repair anti-inflammatory phenotype in response to pathological inflammations.

Selected Publications

View publications for Vincent Hascall, PhD
(Disclaimer: This search is powered by PubMed, a service of the U.S. National Library of Medicine. PubMed is a third-party website with no affiliation with Cleveland Clinic.)

---

Mutoji KN, Sun M, Nash A, Puri S, Hascall V, Coulson-Thomas VJ. Anti-inflammatory protein TNFα-stimulated gene-6 (TSG-6) reduces inflammatory response after brain injury in mice. BMC Immunol. 2021 Aug 4;22(1):52. doi: 10.1186/s12865-021-00443-7. PMID: 34348643; PMCID: PMC8336266.

Misra S, Ghatak S, Moreno-Rodriguez R, Norris R, Hascall V, Markwald R. Periostin/Filamin-A: A Candidate Central Regulatory Axis for Valve Fibrogenesis and Matrix Compaction. Front Cell Dev Biol. 2021 Jun 3;9:649862. doi: 10.3389/fcell.2021.649862. eCollection 2021.

Ghatak S, Hascall VC, Markwald RR, Misra S. FOLFOX Therapy Induces Feedback Upregulation of CD44v6 through YB-1 to Maintain Stemness in Colon Initiating Cells. Int J Mol Sci. 2021 Jan 13;22(2):753. doi: 10.3390/ijms22020753

Midgley AC, Woods EL, Jenkins RH, Brown C, Khalid U, Chavez R, Hascall V, Steadman R, Phillips AO, Meran S. Hyaluronidase-2 Regulates RhoA Signaling, Myofibroblast Contractility, and Other Key Profibrotic Myofibroblast Functions. Am J Pathol. 2020 Jun;190(6):1236-1255. 

Wolk A, Hatipoglu D, Cutler A, Ali M, Bell L, Hua Qi J, Singh R, Batoki J, Karle L, Bonilha VL, Wessely O, Stoehr H, Hascall V, Anand-Apte B. Role of FGF and Hyaluronan in Choroidal Neovascularization in Sorsby Fundus Dystrophy. Cells. 2020 Mar 4;9(3):608. 

Abbadi A, Loftis J, Wang A, Yu M, Wang Y, Shakya S, Li X, Maytin E, Hascall V. Heparin inhibits proinflammatory and promotes anti-inflammatory macrophage polarization under hyperglycemic stress. J Biol Chem. 2020 Apr 10;295(15):4849-4857 

Tseng V, Ni K, Allawzi A, Prohaska C, Hernandez-Lagunas L, Elajaili H, Cali V, Midura R, Hascall V, Triggs-Raine B, Petrache I, Hart CM, Nozik-Grayck E. Extracellular Superoxide Dismutase Regulates Early Vascular Hyaluronan Remodeling in Hypoxic Pulmonary Hypertension. Sci Rep. 2020 Jan 14;10(1):280. 

Caon I, Bartolini B, Moretto P, Parnigoni A, Caravà E, Vitale DL, Alaniz L, Viola M, Karousou E, De Luca G, Hascall VC, Passi A, Vigetti D. Sirtuin 1 reduces hyaluronan synthase 2 expression by inhibiting nuclear translocation of NF-κB and expression of the long-noncoding RNA HAS2-AS1. J Biol Chem. 2020 Mar 13;295(11):3485-3496. 

Sun M, Puri S, Mutoji KN, Coulson-Thomas YM, Hascall VC, Jackson DG, Gesteira TF, Coulson-Thomas VJ. Hyaluronan Derived From the Limbus is a Key Regulator of Corneal Lymphangiogenesis. Invest Ophthalmol Vis Sci. 2019 Mar 1;60(4):1050-1062. 

Ghatak S, Misra S, Moreno-Rodrigue RA, Hascall VC, Leone GW, Markwald RR. Periostin/β1integrin interaction regulates p21-activated kinases in valvular interstitial cell survival and in actin cytoskeleton reorganization. Biochim Biophys Acta Gen Subj. 2019 May;1863(5):813-829.

Hascall VC. The journey of hyaluronan research in the Journal of Biological Chemistry. J Biol Chem 294: 1690-1696, 2019. 

Wang, AJ, Ren J, Abbadi A, Wang, A, Hascall, VC. Heparin affects cytosolic glucose responses of hyperglycemic dividing mesangial cells. J Biol Chem 294:1690-1697, 2019. 

Sun M, Puri S, Mutoji KN, Coulson-Thomas YM, Hascall VC, Jackson DG, Gesteira TF, Coulson-Thomas VJ. Hyaluronan derived from the limbus is a key regulator of corneal lymphangiogenesis. Invest Ophthalmol Vis Sci 60:1050-1062, 2019.

Qadan MA, Piuzzi NS, Boehm C, Nova W, Moos M Jr, Midura RJ, Hascall VC, Malcuit C, Muschler GF. Variation in primary and culture-expanded cells derived from connective tissue progenitors in human bone marrow space, bone trabecula surface and adipose tissue. Cytotherapy. 20:343-360, 2018. 

Sivakumar A, Mahadevan A, Lauer ME, Narvaez RJ, Ramesh S, Demler CM, Souchet NR, Hascall VC, Midura RJ, Garantziotis S, Frank DM, Kimata K, Kurpios NA. Midgut laterality is driven by hyaluronan on the right. Div Cell 10:533-551, 2018.

Viola M, Bartolini B, Vigetti D, Karousou E, Moretto P, Deleonibus S, Sawamura T, Wight TN, Hascall VC, De Luca G, Passi A.  Oxidized low density lipoprotein (LDL) affects hyaluronan synthesis in human aortic smooth muscle cells.  J Biol Chem 2013;288:29595-603. 

Sivakumar A, Mahadevan A, Lauer ME, Narvaez RJ, Ramesh S, Demler CM, Souchet NR, Hascall VC, Midura RJ, Garantziotis S, Frank DB, Kimata K, Kurpios NA. Midgut Laterality Is Driven by Hyaluronan on the Right. Dev Cell. 2018 Sep 10;46(5):533-551.e5.

Qadan MA, Piuzzi NS, Boehm C, Bova W, Moos M Jr, Midura RJ, Hascall VC, Malcuit C, Muschler GF. Variation in primary and culture-expanded cells derived from connective tissue progenitors in human bone marrow space, bone trabecular surface and adipose tissue.  Cytotherapy. 2018 Mar;20(3):343-360. 

Piuzzi NS, Midura RJ, Muschler GF, Hascall VC. Intra-articular hyaluronan injections for the treatment of osteoarthritis: perspective for the mechanism of action. Ther Adv Musculoskelet Dis. 2018 Feb;10(2):55-57 

Midura RJ, Cali V, Lauer ME, Calabro A, Hascall VC. Quantification of hyaluronan (HA) using a simplified fluorophore-assisted carbohydrate electrophoresis (FACE) procedure. Methods Cell Biol. 2018;143:297-316. 

Midgley AC, Oltean S, Hascall V, Woods EL, Steadman R, Phillips AO, Meran S. Nuclear hyaluronidase 2 drives alternative splicing of CD44 pre-mRNA to determine profibrotic or antifibrotic cell phenotype. Sci Signal. 2017 Nov 21;10(506)

Zahreddine HA, Culjkovic-Kraljacic B, Emond A, Pettersson F, Midura R, Lauer M, Del Rincon S, Cali V, Assouline S, Miller WH, Hascall V, Borden KL. The eukaryotic translation initiation factor eIF4E harnesses hyaluronan production to drive its malignant activity. Elife. 2017 Nov 7;6:e29830. 

Gesteira TF, Sun M, Coulson-Thomas YM, Yamaguchi Y, Yeh LK, Hascall V, Coulson-Thomas VJ. Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation. Invest Ophthalmol Vis Sci. 2017 Sep 1;58(11):4407-4421. 

Ghatak S, Markwald RR, Hascall VC, Dowling W, Lottes RG, Baatz JE, Beeson G, Beeson CC, Perrella MA, Thannickal VJ, Misra S. Transforming growth factor β1 (TGFβ1) regulates CD44V6 expression and activity through extracellular signal-regulated kinase (ERK)-induced EGR1 in pulmonary fibrogenic fibroblasts. J Biol Chem. 2017 Jun 23;292(25):10465-10489 

Ghatak S, Hascall VC, Markwald RR, Feghali-Bostwick C, Artlett CM, Gooz M, Bogatkevich GS, Atanelishvili I, Silver RM, Wood J, Thannickal VJ, Misra S. Transforming growth factor β1 (TGFβ1)-induced CD44V6-NOX4 signaling in pathogenesis of idiopathic pulmonary fibrosis. J Biol Chem. 2017 Jun 23;292(25):10490-10519. 

Hascall V, Esko JD. Hyaluronan. 2017. In: Varki A, Cummings RD, Esko JD, Stanley P, Hart GW, Aebi M, Darvill AG, Kinoshita T, Packer NH, Prestegard JH, Schnaar RL, Seeberger PH, editors. Essentials of Glycobiology [Internet]. 3rd edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2015–2017. Chapter 16. 

Dong Y, Arif A, Olsson M, Cali V, Hardman B, Dosanjh M, Lauer M, Midura RJ, Hascall VC, Brown KL, Johnson P. Endotoxin free hyaluronan and hyaluronan fragments do not stimulate TNF-α, interleukin-12 or upregulate co-stimulatory molecules in dendritic cells or macrophages. Sci Rep. 2016 Nov 21;6:36928. 

Coulson-Thomas VJ, Lauer ME, Soleman S, Zhao C, Hascall VC, Day AJ, Fawcett JW. Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Is Constitutively Expressed in Adult Central Nervous System (CNS) and Associated with Astrocyte-mediated Glial Scar Formation following Spinal Cord Injury. J Biol Chem. 2016 Sep 16;291(38):19939-52.

Agre P, Bertozzi C, Bissell M, Campbell KP, Cummings RD, Desai UR, Estes M, Flotte T, Fogleman G, Gage F, Ginsburg D, Gordon JI, Hart G, Hascall V, Kiessling L, Kornfeld S, Lowe J, Magnani J, Mahal LK, Medzhitov R, Roberts RJ, Sackstein R, Sarkar R, Schnaar R, Schwartz N, Varki A, Walt D, Weissman I. Training the next generation of biomedical investigators in glycosciences. J Clin Invest. 2016 Feb;126(2):405-8. 

Vinukonda G, Dohare P, Arshad A, Zia MT, Panda S, Korumilli R, Kayton R, Hascall VC, Lauer ME, Ballabh P. Hyaluronidase and Hyaluronan Oligosaccharides Promote Neurological Recovery after Intraventricular Hemorrhage. J Neurosci. 2016 Jan 20;36(3):872-89. 

Abbadi A, Lauer M, Swaidani S, Wang A, Hascall V. Hyaluronan Rafts on Airway Epithelial Cells. J Biol Chem. 2016 Jan 15;291(3):1448-55. 

Ghatak S, Maytin EV, Mack JA, Hascall VC, Atanelishvili I, Moreno Rodriguez R, Markwald RR, Misra S. Roles of Proteoglycans and Glycosaminoglycans in Wound Healing and Fibrosis. Int J Cell Biol. 2015;2015:834893. 

Misra S, Hascall VC, Atanelishvili I, Moreno Rodriguez R, Markwald RR, Ghatak S. Utilization of Glycosaminoglycans/Proteoglycans as Carriers for Targeted Therapy Delivery. Int J Cell Biol. 2015;2015:537560 

Wang CP, Hascall VC, Zhang F, Linhardt RJ, Abbadi A, Wang A. The Responses of Hyperglycemic Dividing Mesangial Cells to Heparin Are Mediated by the Non-reducing Terminal Trisaccharide. J Biol Chem. 2015 Nov 27;290(48):29045-50. 

Lauer ME, Majors AK, Comhair S, Ruple LM, Matuska B, Subramanian A, Farver C, Dworski R, Grandon D, Laskowski D, Dweik RA, Erzurum SC, Hascall VC, Aronica MA. Hyaluronan and Its Heavy Chain Modification in Asthma Severity and Experimental Asthma Exacerbation. J Biol Chem. 2015 Sep 18;290(38):23124-34. 

Misra S, Hascall VC, Markwald RR, Ghatak S. Interactions between Hyaluronan and Its Receptors (CD44, RHAMM) Regulate the Activities of Inflammation and Cancer. Front Immunol. 2015 May 6;6:201 

Wang A, Sankaranarayanan NV, Yanagishita M, Templeton DM, Desai UR, Sugahara K, Wang CP, Hascall VC. Heparin interaction with a receptor on hyperglycemic dividing cells prevents intracellular hyaluronan synthesis and autophagy responses in models of type 1 diabetes. Matrix Biol. 2015 Oct;48:36-41. 

Midgley AC, Duggal L, Jenkins R, Hascall V, Steadman R, Phillips AO, Meran S. Hyaluronan regulates bone morphogenetic protein-7-dependent prevention and reversal of myofibroblast phenotype. J Biol Chem. 2015 May 1;290(18):11218-34. 

Hascall V, Wang A. Hyperglycemia diverts dividing stem cells to pathological adipogenesis. Stem Cell Res Ther. 2014 Nov 17;5(6):128. 

Lamkin E, Cheng G, Calabro A, Hascall VC, Joo EJ, Li L, Linhardt RJ, Lauer ME. Heavy chain transfer by tumor necrosis factor-stimulated gene 6 to the bikunin proteoglycan. J Biol Chem. 2015 Feb 20;290(8):5156-5166. 

Lauer ME, Loftis J, de la Motte C, Hascall VC. Analysis of the heavy-chain modification and TSG-6 activity in pathological hyaluronan matrices. Methods Mol Biol. 2015;1229:543-8. 

Vigetti D, Deleonibus S, Moretto P, Bowen T, Fischer JW, Grandoch M, Oberhuber A, Love DC, Hanover JA, Cinquetti R, Karousou E, Viola M, D'Angelo ML, Hascall VC, De Luca G, Passi A. Natural antisense transcript for hyaluronan synthase 2 (HAS2-AS1) induces transcription of HAS2 via protein O-GlcNAcylation. J Biol Chem. 2014 Oct 17;289(42):28816-26 

Lauer ME, Hascall VC, Green DE, DeAngelis PL, Calabro A. Irreversible heavy chain transfer to chondroitin. J Biol Chem. 2014 Oct 17;289(42):29171-9. 

Misra S, Ghatak S, Vyas A, O'Brien P, Markwald RR, Khetmalas M, Hascall VC, McCarthy JB, Karamanos NK, Tammi MI, Tammi RH, Prestwitch GD, Padhye S. Isothiocyanate analogs targeting CD44 receptor as an effective strategy against colon cancer. Med Chem Res. 2014 Aug 1;23(8):3836-3851. 

Coulson-Thomas VJ, Gesteira TF, Hascall V, Kao W. Umbilical cord mesenchymal stem cells suppress host rejection: the role of the glycocalyx. J Biol Chem. 2014 Aug 22;289(34):23465-81. 

Wang A, Midura RJ, Vasanji A, Wang AJ, Hascall VC. Hyperglycemia diverts dividing osteoblastic precursor cells to an adipogenic pathway and induces synthesis of a hyaluronan matrix that is adhesive for monocytes. J Biol Chem. 2014 Apr 18;289(16):11410-11420 

Hascall VC, Wang A, Tammi M, Oikari S, Tammi R, Passi A, Vigetti D, Hanson RW, Hart GW. The dynamic metabolism of hyaluronan regulates the cytosolic concentration of UDP-GlcNAc. Matrix Biol. 2014 Apr;35:14-7. 

Wang A, Ren J, Wang CP, Hascall VC. Heparin prevents intracellular hyaluronan synthesis and autophagy responses in hyperglycemic dividing mesangial cells and activates synthesis of an extensive extracellular monocyte-adhesive hyaluronan matrix after completing cell division. J Biol Chem. 2014 Mar 28;289(13):9418-29. 

Ghatak S, Misra S, Norris RA, Moreno-Rodriguez RA, Hoffman S, Levine RA, Hascall VC, Markwald RR. Periostin induces intracellular cross-talk between kinases and hyaluronan in atrioventricular valvulogenesis. J Biol Chem. 2014 Mar 21;289(12):8545-61 

Lauer ME, Aytekin M, Comhair SA, Loftis J, Tian L, Farver CF, Hascall VC, Dweik RA. Modification of hyaluronan by heavy chains of inter-α-inhibitor in idiopathic pulmonary arterial hypertension. J Biol Chem. 2014 Mar 7;289(10):6791-6798. 

Ghatak S, Bogatkevich GS, Atnelishvili I, Akter T, Feghali-Bostwick C, Hoffman S, Fresco VM, Fuchs JC, Visconti RP, Markwald RR, Padhye SB, Silver RM, Hascall VC, Misra S. Overexpression of c-Met and CD44v6 receptors contributes to autocrine TGF-β1 signaling in interstitial lung disease. J Biol Chem. 2014 Mar 14;289(11):7856-72. 

Viola M, Bartolini B, Vigetti D, Karousou E, Moretto P, Deleonibus S, Sawamura T, Wight TN, Hascall VC, De Luca G, Passi A. Oxidized low density lipoprotein (LDL) affects hyaluronan synthesis in human aortic smooth muscle cells. J Biol Chem. 2013 Oct 11;288(41):29595-603. 

Lauer ME, Glant TT, Mikecz K, DeAngelis PL, Haller FM, Husni ME, Hascall VC, Calabro A. Irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6. J Biol Chem. 2013 Jan 4;288(1):205-14. 

Lauer ME, Cheng G, Swaidani S, Aronica MA, Weigel PH, Hascall VC. Tumor necrosis factor-stimulated gene-6 (TSG-6) amplifies hyaluronan synthesis by airway smooth muscle cells. J Biol Chem. 2013 Jan 4;288(1):423-31 

Swaidani S, Cheng G, Lauer ME, Sharma M, Mikecz K, Hascall VC, Aronica MA. TSG-6 protein is crucial for the development of pulmonary hyaluronan deposition, eosinophilia, and airway hyperresponsiveness in a murine model of asthma. J Biol Chem. 2013 Jan 4;288(1):412-22. 

Caralla T, Joshi P, Fleury S, Luangphakdy V, Shinohara K, Pan H, Boehm C, Vasanji A, Hefferan TE, Walker E, Yaszemski M, Hascall V, Zborowski M, Muschler GF. In vivo transplantation of autogenous marrow-derived cells following rapid intraoperative magnetic separation based on hyaluronan to augment bone regeneration. Tissue Eng Part A. 2013 Jan;19(1-2):125-34. 

Cheng G, Swaidani S, Sharma M, Lauer ME, Hascall VC, Aronica MA. Correlation of hyaluronan deposition with infiltration of eosinophils and lymphocytes in a cockroach-induced murine model of asthma. Glycobiology. 2013 Jan;23(1):43-58.

Lauer ME, Glant TT, Mikecz K, DeAngelis PL, Haller FM, Husni ME, Hascall VC, Calabro A. Irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6.  J Biol Chem  2013;288:205-14. doi: 10.1074/jbc.M112.403998. PMCID: PMC3537015.

Lauer ME, Cheng G, Swaidani S, Aronica MA, Weigel PH, Hascall VC. Tumor necrosis factor-stimulated gene-6 (TSG-6) amplifies hyaluronan synthesis by airway smooth muscle cells. J Biol Chem 2013;288:423-31. doi: 10.1074/jbc.M112.389882. PMCID: PMC3537040.

Swaidani S, Cheng G, Lauer ME, Sharma M, Mikecz K, Hascall VC, Aronica MA. TSG-6 protein is crucial for the development of pulmonary hyaluronan deposition, eosinophilia, and airway hyperresponsiveness in a murine model of asthma. J Biol Chem 2013;288:412-22. doi: 10.1074/jbc.M112.389874. PMCID: PMC3537038.

Vigetti D, Deleonibus S, Moretto P, Karousou E, Viola M, Bartolini B, Hascall VC, Tammi M, De Luca G, Passi A. Role of UDP-N-acetylglucosamine (GlcNAc) and O-GlcNAcylation of hyaluronan synthase 2 in the control of chondroitin sulfate and hyaluronan synthesis. J Biol Chem. 2012 Oct 12;287(42):35544-35555. 

Kouvidi K, Berdiaki A, Nikitovic D, Katonis P, Afratis N, Hascall VC, Karamanos NK, Tzanakakis GN. Role of receptor for hyaluronic acid-mediated motility (RHAMM) in low molecular weight hyaluronan (LMWHA)-mediated fibrosarcoma cell adhesion. J Biol Chem. 2011 Nov 4;286(44):38509-38520. 

Mack JA, Feldman RJ, Itano N, Kimata K, Lauer M, Hascall VC, Maytin EV. Enhanced inflammation and accelerated wound closure following tetraphorbol ester application or full-thickness wounding in mice lacking hyaluronan synthases Has1 and Has3. J Invest Dermatol. 2012 Jan;132(1):198-207. 

Bulek K, Liu C, Swaidani S, Wang L, Page RC, Gulen MF, Herjan T, Abbadi A, Qian W, Sun D, Lauer M, Hascall V, Misra S, Chance MR, Aronica M, Hamilton T, Li X. The inducible kinase IKKi is required for IL-17-dependent signaling associated with neutrophilia and pulmonary inflammation. Nat Immunol. 2011 Aug 7;12(9):844-52. 

Vigetti D, Rizzi M, Moretto P, Deleonibus S, Dreyfuss JM, Karousou E, Viola M, Clerici M, Hascall VC, Ramoni MF, De Luca G, Passi A. Glycosaminoglycans and glucose prevent apoptosis in 4-methylumbelliferone-treated human aortic smooth muscle cells. J Biol Chem. 2011 Oct 7;286(40):34497-503. 

Nagyeri G, Radacs M, Ghassemi-Nejad S, Tryniszewska B, Olasz K, Hutas G, Gyorfy Z, Hascall VC, Glant TT, Mikecz K. TSG-6 protein, a negative regulator of inflammatory arthritis, forms a ternary complex with murine mast cell tryptases and heparin. J Biol Chem. 2011 Jul 1;286(26):23559-69. doi: 10.1074/jbc.M111.222026. 

Misra S, Heldin P, Hascall VC, Karamanos NK, Skandalis SS, Markwald RR, Ghatak S. Hyaluronan-CD44 interactions as potential targets for cancer therapy. FEBS J. 2011 May;278(9):1429-43. doi: 10.1111/j.1742-4658.2011.08071.x. 

Wang A, de la Motte C, Lauer M, Hascall V. Hyaluronan matrices in pathobiological processes. FEBS J. 2011 May;278(9):1412-8. doi: 10.1111/j.1742-4658.2011.08069.x. 

Hascall V, Karamanos N. Regulatory roles of hyaluronan in health and disease. FEBS J. 2011 May;278(9):1411. doi: 10.1111/j.1742-4658.2011.08068.x. 

Cheng G, Swaidani S, Sharma M, Lauer ME, Hascall VC, Aronica MA. Hyaluronan deposition and correlation with inflammation in a murine ovalbumin model of asthma. Matrix Biol. 2011 Mar;30(2):126-34. doi: 10.1016/j.matbio.2010.12.003. 

Vigetti D, Clerici M, Deleonibus S, Karousou E, Viola M, Moretto P, Heldin P, Hascall VC, De Luca G, Passi A. Hyaluronan synthesis is inhibited by adenosine monophosphate-activated protein kinase through the regulation of HAS2 activity in human aortic smooth muscle cells. J Biol Chem. 2011 Mar 11;286(10):7917-7924. doi: 10.1074/jbc.M110.193656.

Carrino DA, Calabro A, Darr AB, Dours-Zimmermann MT, Sandy JD, Zimmermann DR, Sorrell JM, Hascall VC, Caplan AI. Age-related differences in human skin proteoglycans. Glycobiology. 2011 Feb;21(2):257-68. doi: 10.1093/glycob/cwq162. 

Vigetti D, Genasetti A, Karousou E, Viola M, Moretto P, Clerici M, Deleonibus S, De Luca G, Hascall VC, Passi A. Proinflammatory cytokines induce hyaluronan synthesis and monocyte adhesion in human endothelial cells through hyaluronan synthase 2 (HAS2) and the nuclear factor-kappaB (NF-kappaB) pathway. J Biol Chem. 2010 Aug 6;285(32):24639-45. doi: 10.1074/jbc.M110.134536. 

Zhu L, Zhuo L, Kimata K, Yamaguchi E, Watanabe H, Aronica MA, Hascall VC, Baba K. Deficiency in the serum-derived hyaluronan-associated protein-hyaluronan complex enhances airway hyperresponsiveness in a murine model of asthma. Int Arch Allergy Immunol. 2010;153(3):223-33. doi: 10.1159/000314362. 

Ghatak S, Hascall VC, Markwald RR, Misra S. Stromal hyaluronan interaction with epithelial CD44 variants promotes prostate cancer invasiveness by augmenting expression and function of hepatocyte growth factor and androgen receptor. J Biol Chem. 2010 Jun 25;285(26):19821-32. doi: 10.1074/jbc.M110.104273. 

Vigetti D, Genasetti A, Karousou E, Viola M, Clerici M, Bartolini B, Moretto P, De Luca G, Hascall VC, Passi A. Modulation of hyaluronan synthase activity in cellular membrane fractions. J Biol Chem. 2009 Oct 30;284(44):30684-94. doi: 10.1074/jbc.M109.040386. 

Wang A, Hascall VC. Hyperglycemia, intracellular hyaluronan synthesis, cyclin D3 and autophagy. Autophagy. 2009 Aug;5(6):864-5. doi: 10.4161/auto.9041. 

Nigro J, Wang A, Mukhopadhyay D, Lauer M, Midura RJ, Sackstein R, Hascall VC. Regulation of heparan sulfate and chondroitin sulfate glycosaminoglycan biosynthesis by 4-fluoro-glucosamine in murine airway smooth muscle cells. J Biol Chem. 2009 Jun 19;284(25):16832-16839. doi: 10.1074/jbc.M109.002956. 

Ren J, Hascall VC, Wang A. Cyclin D3 mediates synthesis of a hyaluronan matrix that is adhesive for monocytes in mesangial cells stimulated to divide in hyperglycemic medium. J Biol Chem. 2009 Jun 12;284(24):16621-16632. doi: 10.1074/jbc.M806430200. 

Misra S, Hascall VC, De Giovanni C, Markwald RR, Ghatak S. Delivery of CD44 shRNA/nanoparticles within cancer cells: perturbation of hyaluronan/CD44v6 interactions and reduction in adenoma growth in Apc Min/+ MICE. J Biol Chem. 2009 May 1;284(18):12432-46. doi: 10.1074/jbc.M806772200. 

Vigetti D, Rizzi M, Viola M, Karousou E, Genasetti A, Clerici M, Bartolini B, Hascall VC, De Luca G, Passi A. The effects of 4-methylumbelliferone on hyaluronan synthesis, MMP2 activity, proliferation, and motility of human aortic smooth muscle cells. Glycobiology. 2009 May;19(5):537-46. doi: 10.1093/glycob/cwp022. 

Lauer ME, Mukhopadhyay D, Fulop C, de la Motte CA, Majors AK, Hascall VC. Primary murine airway smooth muscle cells exposed to poly(I,C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix. J Biol Chem. 2009 Feb 20;284(8):5299-312. doi: 10.1074/jbc.M807965200. 

Lauer ME, Fulop C, Mukhopadhyay D, Comhair S, Erzurum SC, Hascall VC. Airway smooth muscle cells synthesize hyaluronan cable structures independent of inter-alpha-inhibitor heavy chain attachment. J Biol Chem. 2009 Feb 20;284(8):5313-23. doi: 10.1074/jbc.M807979200. 

Bandyopadhyay SK, de la Motte CA, Kessler SP, Hascall VC, Hill DR, Strong SA. Hyaluronan-mediated leukocyte adhesion and dextran sulfate sodium-induced colitis are attenuated in the absence of signal transducer and activator of transcription 1. Am J Pathol. 2008 Nov;173(5):1361-8. doi: 10.2353/ajpath.2008.080444. 

Aytekin M, Comhair SA, de la Motte C, Bandyopadhyay SK, Farver CF, Hascall VC, Erzurum SC, Dweik RA. High levels of hyaluronan in idiopathic pulmonary arterial hypertension. Am J Physiol Lung Cell Mol Physiol. 2008 Nov;295(5):L789-99. doi: 10.1152/ajplung.90306.2008. 

Misra S, Hascall VC, Berger FG, Markwald RR, Ghatak S. Hyaluronan, CD44, and cyclooxygenase-2 in colon cancer. Connect Tissue Res. 2008;49(3):219-24. doi: 10.1080/03008200802143356. 

Jokela TA, Lindgren A, Rilla K, Maytin E, Hascall VC, Tammi RH, Tammi MI. Induction of hyaluronan cables and monocyte adherence in epidermal keratinocytes. Connect Tissue Res. 2008;49(3):115-9. doi: 10.1080/03008200802148439. 

Lauer ME, Erzurum SC, Mukhopadhyay D, Vasanji A, Drazba J, Wang A, Fulop C, Hascall VC. Differentiated murine airway epithelial cells synthesize a leukocyte-adhesive hyaluronan matrix in response to endoplasmic reticulum stress. J Biol Chem. 2008 Sep 19;283(38):26283-96. doi: 10.1074/jbc.M803350200. 

Lewis A, Steadman R, Manley P, Craig K, de la Motte C, Hascall V, Phillips AO. Diabetic nephropathy, inflammation, hyaluronan and interstitial fibrosis. Histol Histopathol. 2008 Jun;23(6):731-9. doi: 10.14670/HH-23.731. 

Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A. Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging. J Biol Chem. 2008 Feb 15;283(7):4448-58. doi: 10.1074/jbc.M709051200. Epub 2007 Dec 12. 

Pasonen-Seppänen SM, Maytin EV, Törrönen KJ, Hyttinen JM, Hascall VC, MacCallum DK, Kultti AH, Jokela TA, Tammi MI, Tammi RH. All-trans retinoic acid-induced hyaluronan production and hyperplasia are partly mediated by EGFR signaling in epidermal keratinocytes. J Invest Dermatol. 2008 Apr;128(4):797-807. doi: 10.1038/sj.jid.5701098. 

Lauer ME, Hascall VC, Wang A. Heparan sulfate analysis from diabetic rat glomeruli. J Biol Chem. 2007 Jan 12;282(2):843-52. doi: 10.1074/jbc.M608823200. 

Selbi W, Day AJ, Rugg MS, Fülöp C, de la Motte CA, Bowen T, Hascall VC, Phillips AO. Overexpression of hyaluronan synthase 2 alters hyaluronan distribution and function in proximal tubular epithelial cells. J Am Soc Nephrol. 2006 Jun;17(6):1553-67. doi: 10.1681/ASN.2005080879. 

Vigetti D, Ori M, Viola M, Genasetti A, Karousou E, Rizzi M, Pallotti F, Nardi I, Hascall VC, De Luca G, Passi A. Molecular cloning and characterization of UDP-glucose dehydrogenase from the amphibian Xenopus laevis and its involvement in hyaluronan synthesis. J Biol Chem. 2006 Mar 24;281(12):8254-63. doi: 10.1074/jbc.M508516200 

Mack JA, Li L, Sato N, Hascall VC, Maytin EV. Hoxb13 up-regulates transglutaminase activity and drives terminal differentiation in an epidermal organotypic model. J Biol Chem. 2005 Aug 19;280(33):29904-11. doi: 10.1074/jbc.M505262200 

Gilbert RJ, McKeon RJ, Darr A, Calabro A, Hascall VC, Bellamkonda RV. CS-4,6 is differentially upregulated in glial scar and is a potent inhibitor of neurite extension. Mol Cell Neurosci. 2005 Aug;29(4):545-58. doi: 10.1016/j.mcn.2005.04.006. 

Rugg MS, Willis AC, Mukhopadhyay D, Hascall VC, Fries E, Fülöp C, Milner CM, Day AJ. Characterization of complexes formed between TSG-6 and inter-alpha-inhibitor that act as intermediates in the covalent transfer of heavy chains onto hyaluronan. J Biol Chem. 2005 Jul 8;280(27):25674-86. doi: 10.1074/jbc.M501332200. 

Zhang XL, Selbi W, de la Motte C, Hascall V, Phillips AO. Bone morphogenic protein-7 inhibits monocyte-stimulated TGF-beta1 generation in renal proximal tubular epithelial cells. J Am Soc Nephrol. 2005 Jan;16(1):79-89. doi: 10.1681/ASN.2004050395. 

Karousou EG, Militsopoulou M, Porta G, De Luca G, Hascall VC, Passi A. Polyacrylamide gel electrophoresis of fluorophore-labeled hyaluronan and chondroitin sulfate disaccharides: application to the analysis in cells and tissues. Electrophoresis. 2004 Sep;25(17):2919-25. doi: 10.1002/elps.200406034.

Zhang XL, Selbi W, de la Motte C, Hascall V, Phillips A. Renal proximal tubular epithelial cell transforming growth factor-beta1 generation and monocyte binding. Am J Pathol. 2004 Sep;165(3):763-73. doi: 10.1016/s0002-9440(10)63339-1. 

Straach KJ, Shelton JM, Richardson JA, Hascall VC, Mahendroo MS. Regulation of hyaluronan expression during cervical ripening. Glycobiology. 2005 Jan;15(1):55-65. doi: 10.1093/glycob/cwh137 

Hascall VC, Majors AK, De La Motte CA, Evanko SP, Wang A, Drazba JA, Strong SA, Wight TN. Intracellular hyaluronan: a new frontier for inflammation? Biochim Biophys Acta. 2004 Jul 6;1673(1-2):3-12. doi: 10.1016/j.bbagen.2004.02.013. 

Zhuo L, Hascall VC, Kimata K. Inter-alpha-trypsin inhibitor, a covalent protein-glycosaminoglycan-protein complex. J Biol Chem. 2004 Sep 10;279(37):38079-82. doi: 10.1074/jbc.R300039200. 

Passi A, Sadeghi P, Kawamura H, Anand S, Sato N, White LE, Hascall VC, Maytin EV. Hyaluronan suppresses epidermal differentiation in organotypic cultures of rat keratinocytes. Exp Cell Res. 2004 Jun 10;296(2):123-34. doi: 10.1016/j.yexcr.2004.01.031. 

Selbi W, de la Motte C, Hascall V, Phillips A. BMP-7 modulates hyaluronan-mediated proximal tubular cell-monocyte interaction. J Am Soc Nephrol. 2004 May;15(5):1199-211. doi: 10.1097/01.asn.0000125619.27422.8e. 

Grande-Allen KJ, Calabro A, Gupta V, Wight TN, Hascall VC, Vesely I. Glycosaminoglycans and proteoglycans in normal mitral valve leaflets and chordae: association with regions of tensile and compressive loading. Glycobiology. 2004 Jul;14(7):621-33. doi: 10.1093/glycob/cwh076. 

Wang A, Hascall VC. Hyaluronan structures synthesized by rat mesangial cells in response to hyperglycemia induce monocyte adhesion. J Biol Chem. 2004 Mar 12;279(11):10279-85. doi: 10.1074/jbc.M312045200. 

Majors AK, Austin RC, de la Motte CA, Pyeritz RE, Hascall VC, Kessler SP, Sen G, Strong SA.Endoplasmic reticulum stress induces hyaluronan deposition and leukocyte adhesion. J Biol Chem. 2003 Nov 21;278(47):47223-31. doi: 10.1074/jbc.M304871200. 

de la Motte CA, Hascall VC, Drazba J, Bandyopadhyay SK, Strong SA. Mononuclear leukocytes bind to specific hyaluronan structures on colon mucosal smooth muscle cells treated with polyinosinic acid:polycytidylic acid: inter-alpha-trypsin inhibitor is crucial to structure and function. Am J Pathol. 2003 Jul;163(1):121-33. doi: 10.1016/s0002-9440(10)63636-x. 

Mack JA, Abramson SR, Ben Y, Coffin JC, Rothrock JK, Maytin EV, Hascall VC, Largman C, Stelnicki EJ. Hoxb13 knockout adult skin exhibits high levels of hyaluronan and enhanced wound healing. FASEB J. 2003 Jul;17(10):1352-4. doi: 10.1096/fj.02-0959fje. 

Gordon LB, Harten IA, Calabro A, Sugumaran G, Csoka AB, Brown WT, Hascall V, Toole BP. Hyaluronan is not elevated in urine or serum in Hutchinson-Gilford Progeria Syndrome. Hum Genet. 2003 Jul;113(2):178-87. doi: 10.1007/s00439-003-0958-9. 

Fülöp C, Szántó S, Mukhopadhyay D, Bárdos T, Kamath RV, Rugg MS, Day AJ, Salustri A, Hascall VC, Glant TT, Mikecz K. Impaired cumulus mucification and female sterility in tumor necrosis factor-induced protein-6 deficient mice. Development. 2003 May;130(10):2253-61. doi: 10.1242/dev.00422. 

Knepper MA, Saidel GM, Hascall VC, Dwyer T. Concentration of solutes in the renal inner medulla: interstitial hyaluronan as a mechano-osmotic transducer. Am J Physiol Renal Physiol. 2003 Mar;284(3):F433-46. doi: 10.1152/ajprenal.00067.2002. 

Calabro A, Oken MM, Hascall VC, Masellis AM. Characterization of hyaluronan synthase expression and hyaluronan synthesis in bone marrow mesenchymal progenitor cells: predominant expression of HAS1 mRNA and up-regulated hyaluronan synthesis in bone marrow cells derived from multiple myeloma patients. Blood. 2002 Oct 1;100(7):2578-85. doi: 10.1182/blood-2002-01-0030. 

Seidler DG, Breuer E, Grande-Allen KJ, Hascall VC, Kresse H. Core protein dependence of epimerization of glucuronosyl residues in galactosaminoglycans. J Biol Chem. 2002 Nov 1;277(44):42409-16. doi: 10.1074/jbc.M208442200. 

Toole BP, Hascall VC. Hyaluronan and tumor growth. Am J Pathol. 2002 Sep;161(3):745-7. doi: 10.1016/S0002-9440(10)64232-0. 

Rilla K, Lammi MJ, Sironen R, Törrönen K, Luukkonen M, Hascall VC, Midura RJ, Hyttinen M, Pelkonen J, Tammi M, Tammi R. Changed lamellipodial extension, adhesion plaques and migration in epidermal keratinocytes containing constitutively expressed sense and antisense hyaluronan synthase 2 (Has2) genes. J Cell Sci. 2002 Sep 15;115(Pt 18):3633-43. doi: 10.1242/jcs.00042. 

McDonald J, Hascall VC. Hyaluronan minireview series. J Biol Chem. 2002 Feb 15;277(7):4575-9. doi: 10.1074/jbc.R100064200.