Our purpose is to provide contact information and technical help to the department and to introduce new Techs to the people and resources available within the department and institution. We want all of the members of the Department of Cell Biology fellows, students and faculty to know what kind of expertise each of us has in case anyone needs technical help. We consider ourselves an essential component of the education and training capacity within Cell Biology and all of us want to be helpful.

In addition we oversee the maintenance of shared equipment in the Cell Biology Department. You will find a separate web page for equipment and the contact info for the Techs who can help troubleshoot or contact repair personnel. If you would like any information about the Techs or about these web pages, feel free to contact any member of the current Tech Executive Committee: Pat DiBello (current Chair), Diane Greene, Mark Calabro, Jennifer Major and Greg Brubaker. Also please send us suggestions for improving the usefulness of our website.
To use this Section effectively, use "Find" under the "Edit" Menu above for the type of Expertise in which you are interested. Let us know if you have a suggestion for making this Section more valuable to users.
| ANIMAL MODELS | |||
| Mouse/Rat Models of Cardiovascular Disease | Jennifer Major | 55603 | |
| Matt Kiedrowski | 55603 | ||
| Mouse Model of Chronic Renal Failure | Angela Miniard | 59757 | |
| Rat Models of Hypertension | Pat DiBello | 42916 | |
| Other Animal Models | Dave Schmitt | 56466 | |
| CELL/TISSUE CULTURE | |||
| Primary Monocyte/macrophages and/or Macrophage Cell Lines | |||
| Various Mouse Macrophage Lines | Greg Brubaker | 43723 | |
| Primaries, U937, MonoMac6 | Suman Kundu | 45508 | |
| Primaries, U937, THP1 | Pat DiBello | 42916 | |
| Primaries | Jennifer Major | 55603 | |
| J774 | Diane Greene | 49173 | |
| Rat, Human Macrophages | Dave Schmitt | 56466 | |
| Primary Endothelial Cells and/or Cell Lines (HAEC, HUVEC, BAEC, etc) | |||
| Pat DiBello | 42916 | ||
| Dave Schmitt | 56466 | ||
| Bacterial Cell Lines | Greg Brubaker | 43723 | |
| Dave Schmitt | 56466 | ||
| Insect Cell Lines | Pat DiBello | 42916 | |
| Other Cell lines | |||
| Hybridomas | Pat DiBello | 42916 | |
| Smooth Muscle | Pat DiBello | 42916 | |
| Fibroblast | Pat DiBello | 42916 | |
| Hep G2 | Diane Greene | 49173 | |
| Pat DiBello | 42916 | ||
| Y1 | Diane Greene | 49173 | |
| CHO | " | " | |
| SW872 | " | " | |
| BECKMAN COULTER COUNTER | Greg Brubaker | 43723 | |
| Shirley Mann | 51900 | ||
| Dave Schmitt | 56466 | ||
| HISTOPATHOLOGY | Colin Agatista-Boyle | 43723 | |
| Xiaorong Zhou | 56784 | ||
| FLUORESCENCE MICROSCOPY | Lucila Licate | 59913 | |
| Greg Brubaker | 43723 | ||
| Shirley Mann | 51900 | ||
| Dave Schmitt | 56466 | ||
| PROTEIN and/or LIPID CHEMISTRY | |||
| Lipid / Oxidized Lipid / Lipoprotein Chemistry | Diane Greene | 49173 | |
| Dave Schmitt | 56466 | ||
| Xiaoming Fu | 56466 | ||
| rec Protein Expression | |||
| Pat DiBello | 42916 | ||
| Protein Purification | Diane Greene | 49173 | |
| Pat DiBello | 42916 | ||
| Greg Brubaker | 43723 | ||
| Shirley Mann | 51900 | ||
| Matt Kiedrowski | 49139 | ||
| Dave Schmitt | 56466 | ||
| Immunoprecipitation | Pat DiBello | 42916 | |
| ELISA | Pat DiBello | 42916 | |
| Angela Miniard | 59757 | ||
| Dave Schmitt | 56466 | ||
| Enzyme Kinetics | Pat DiBello | 42916 | |
| Greg Brubaker | 43723 | ||
| Dave Schmitt | 56466 | ||
| Ultracentrifugation | Diane Greene | 49173 | |
| Greg Brubaker | 43723 | ||
| Radiolabeling | Pat DiBello | 42916 | |
| Diane Greene | 49173 | ||
| Dave Schmitt | 56466 | ||
| Biotinylation | Pat DiBello | 42916 | |
| Radio-ligand Binding | Pat DiBello | 42916 | |
| CHROMATOGRAPHY | |||
| Affinity | |||
| GST, His-Tag, Amylose-MBP | Greg Brubaker | 43723 | |
| Protein A/G | Pat DiBello | 42916 | |
| GST, His-Tag | Dave Schmitt | 56466 | |
| Ion-Exchange Hydroxyapatite | Greg Brubaker | 43723 | |
| Pat DiBello | 42916 | ||
| Diane Greene | 49173 | ||
| Gel Filtration | Greg Brubaker | 43723 | |
| Pat DiBello | 42916 | ||
| Diane Greene | 49173 | ||
| Dave Schmitt | 56466 | ||
| HPLC | Dave Schmitt | 56466 | |
| Pat DiBello | 42916 | ||
| Xiaoming Fu | 56466 | ||
| GC | Shirley Mann | 51900 | |
| Xiaoming Fu | 56466 | ||
| TLC | Diane Greene | 49173 | |
| Shirley Mann | 51900 | ||
| Dave Schmitt | 56466 | ||
| Xiaoming Fu | 56466 | ||
| ANALYTICAL CHEMISTRY | |||
| UV-Vis Spectrophotometry | ALL | ||
| Organic Extractions | Diane Greene | 49173 | |
| Shirley Mann | 51900 | ||
| Organic Synthesis of Amino Acids & peptides | Xiaoming Fu | 56466 | |
| Solid Phase Extractions | Shirley Mann | 51900 | |
| Scintillation and/or Gamma Counting | Greg Brubaker | 43723 | |
| Pat DiBello | 42916 | ||
| Diane Greene | 49173 | ||
| Shirley Mann | 51900 | ||
| Dave Schmitt | 56466 | ||
| Fluorescence Plate Reader | Greg Brubaker | 43723 | |
| Armend Axhemi | 48339 | ||
| Pat DiBello | 42916 | ||
| Shirley Mann | 51900 | ||
| Dave Schmitt | 56466 | ||
| Fluorimeters | Diane Greene | 49173 | |
| Diane Greene | 49173 | ||
| Mass Spectrometry | Xiaoming Fu | 56466 | |
| NMR and IR | Xiaoming Fu | 56466 | |
| QC & QA | Shirley Mann | 51900 | |
| Flame Photometry | Shirley Mann | 51900 | |
| Chemistry Autoanalyzers | Shirley Mann | 51900 | |
| Milli-Q Water System | Dave Schmitt | 56466 | |
| Speed Vac | Dave Schmitt | 56466 | |
| Pat DiBello | 42916 |
||
| Lyophilizer | Armend Axhemi | 48339 | |
| Dave Schmitt | 56466 | ||
| Sonicator | Pat DiBello | 42916 | |
| Armend Axhemi | 48339 |
||
| ELECTROPHORESIS | |||
| PAGE | Greg Brubaker | 43723 | |
| Pat DiBello | 42916 | ||
| Diane Greene | 49173 | ||
| Dave Schmitt | 56466 | ||
| Jennifer Major | 55603 | ||
| DNA Sequencing | Dave Schmitt | 56466 | |
| Greg Brubaker | 43723 | ||
| Agarose | Greg Brubaker | 43723 | |
| Shirley Mann | 51900 | ||
| Dave Schmitt | 56466 | ||
| Capillary | Greg Brubaker | 43723 | |
| Shirley Mann | 51900 | ||
| IEF | Pat DiBello | 42916 | |
| 2D | Pat DiBello | 42916 | |
| Western Blot | Pat DiBello | 42916 | |
| Diane Greene | 49173 | ||
| Jennifer Major | 55603 | ||
| Northern Blot | Lori Mavrakis | 41220 | |
| Angela Miniard | 59757 | ||
| Jennifer Major | 55603 | ||
| Dave Schmitt | 56466 | ||
| Southern Blot | Jennifer Major | 55603 | |
| Gel Shift | Dave Schmitt | 56466 | |
| Gel Documentaion | |||
| UV & Chemiluminescence | Greg Brubaker | 43723 | |
| UV & Chemiluminescence | Shirley Mann | 51900 | |
| Phosphorimager | Pat DiBello | 42916 | |
| UV & Chemiluminescence | Dave Schmitt | 56466 | |
| Densitometry | Pat DiBello | 42916 | |
| MOLECULAR BIOLOGY | |||
| DNA Isolation & Purification | Greg Brubaker | 43723 | |
| Shirley Mann | 51900 | ||
| Dave Schmitt | 56466 | ||
| Cloning | |||
| Greg Brubaker | 43723 | ||
| Jennifer Major | 55603 | ||
| Dave Schmitt | 56466 | ||
| DNA Labeling | Jennifer Major | 55603 | |
| DNA Transfection | Dave Schmitt | 56466 | |
| Jennifer Major | 55603 | ||
| Oligo Design | Jennifer Major | 55603 | |
| Transformation | |||
| Dave Schmitt | 56466 | ||
| Greg Brubaker | 43723 | ||
| PCR | |||
| Greg Brubaker | 43723 | ||
| Jennifer Major | 55603 | ||
| Shirley Mann | 51900 | ||
| Dave Schmitt | 56466 | ||
| Luciferase Assay | Greg Brubaker | 43723 | |
| Dave Schmitt | 56466 | ||
| Affymetrix Gene Chip | Angela Miniard | 59757 | |
| RNA Isolation & Purification | Jennifer Major | 55603 | |
| Angela Miniard | 59757 | ||
| RT-PCR | Jennifer Major | 55603 | |
| Angela Miniard | 59757 | ||
| Nuclear Run-on | Jennifer Major | 55603 | |
| rec Protein Expression | Pat DiBello | 42916 | |
PLEASE NOTE: The use of this equipment
is by RESERVATION ONLY!
Please familiarize yourself with
its operation prior to use. The manuals for the equipment are located either
near the instrument or in a labeled file cabinet in NC1-140. The contact person
is only responsible for contacting the service provider for repairs and not
for individual training; however they may be able to help you locate the manuals
or find someone else who is a frequent users of that specific piece of equipment.
It is your responsibility to bring all of the consumables with you and to
clean up after yourself.
| Equipment Name | Location | Contact for help | Vendor | Ext |
|---|---|---|---|---|
| NC Common Equipment | ||||
| Aggregometer | NC1-137 | Jennifer Major David Kennedy |
Choro-Log | 55603 55603 |
| ABI Real Time PCR | NC1-112 | Armend Axhemi Maryam Zamanian-Daryoush |
ABI | 48339 51900 |
| Centrifuges (ALL Beckman) | NC1-Hallway | Diane Greene Mette Johansen |
Beckman | 49173 41228 |
| Allegra 25R Centrifuge | NC1-Hallway | Greg Brubaker Weihuan Wang |
Beckman | 43723 52686 |
| Discovery M150 Mini Ultracentrifuge | NC1-Hallway | Prabar Ghosh Greg Brubaker |
Sorvall (Thermo Scientific) | 41229 43723 |
| Tri-Carb 2100 Scintillation Counter | NC1-Hallway | Diane Greene David Kennedy |
Perkin Elmer (Packard) | 49173 55603 |
| Tri-Carb 1900 Scintillation Counter | NC1-Hallway | Diane Greene David Kennedy |
Perkin Elmer (Packard) | 49173 55603 |
| Gamma Counter 5000 | NC1-Hallway | Diane Greene David Kennedy |
Perkin Elmer (Packard) | 49173 55603 |
| Flammable Cabinets | NC1-Hallway | Jennifer Major Prabar Ghosh |
55603 41229 |
|
| Gemini SpectraMax microplate ELISA plate reader | NC1-140 | Phil Klenotic Mike Berk |
Molecular Devices | 55603 45932 |
| Gemini EM SpectraMax microplate Fluorimeter | NC1-140 | Marharyta Yakutovich Junzhen Zhang |
Molecular Devices | 41029 49342 |
| Leica Microscope-DMLB | NC1-121 | Lucila Licate Venk Betapudi |
Leica | 59913 53699 |
| Leica DMIRE-2 Microscope | NC1-112 | Lucila Licate Jordan Beach |
Leica | 59913 59913 |
| Gel Imaging System | NC1-140 | Jennifer Major Jason Ospina |
Kodak | 55603 49342 |
| GE ImageQuant ECL | NC1-112 | Angela Miniard Mike Budiman |
GE | 59757 59757 |
| Guava Cell Counter | NC1-113 | Jennifer Major David Kennedy |
Guava | 55603 55603 |
| Hypoxia Chamber | NC1-Hallway | Prabar Ghosh | 41229 | |
| Incubator Shaker 4300 | NC1-Hallway | Lucila Licate Jie Jae |
New Brunswick | 59913 41214 |
| Incubator Shaker I-2500 | NC1-Hallway | Peter Dy Jie Jae |
New Brunswick | 52686 41214 |
| LS-50B Luminometer | NC1-140 | Rick Morton Jodi Bubenik |
Perkin-Elmer | 45850 45850 |
| Lyophilizer | NC1-Hallway | Lisa Dechert Weifei Zhu |
Labconco | 45855 55603 |
| N2/CO2/Vac | NC1-Hallway | Jennifer Major Prabar Ghosh |
55603 41229 |
|
| Microplate Luminometer 1420 | NC1-107 | Angela Miniard Sanghamitra Bhattacharyya |
Perkin Elmer | 59757 59757 |
| IMAC/ Microtek Scanner | NC1-126B | Abul Arif | Microtek | 41229 |
| Milli Q Water Purification System & | NC1-122 | Kristen Nowak Yi Fan |
Millipore | 55603 41229 |
| NC Cold Room | NC1-108 | Peter Dy Pallavi Bhattaram |
52686 52686 |
|
| NC Cold Room | NC1-123 | Kristen Nowak Bin Ren |
55603 41228 |
|
| DNA 110 Speed Vac | NC1-Hallway | Pat DiBello Fulvia Terenzi |
Thermo Savant | 42916 41214 |
| DNA 110 Speed Vac | NC1-112 | Pat DiBello Fulvia Terenzi |
Thermo Savant | 42916 41214 |
| Shimadzu Spectrophotometer UV-160U | NC1-112 | Mette Johansen Lucila Licate |
Shimadzu | 41228 59913 |
| NN Common Equipment | ||||
| Centrifuge J2-HS | NN1-Hallway | Pat DiBello Peggy Robinet |
Beckman | 42916 43723 |
| Ultra-Centrifuge L8-80M | NN1-Hallway | Pat DiBello Peggy Robinet |
Beckman | 42916 43723 |
| Gamma Counter 4000 | NN1-Hallway | Pat DiBello Peggy Robinet |
Beckman | 42916 43723 |
| NN Dark Room | NN1-Hallway | Suman Kundu Fabio Fonseca |
45508 58184 |
|
| Fluorimeter (BioTek Synergy HT) | NN1-27A | Armend Axhemi Ashish Bhattacharjee |
BioTek | 48339 45508 |
| Incubator/Shaker (ThermoMax Q5000) | NN1-Hallway | Armend Axhemi Fabio Fonseca |
Thermo | 48339 58184 |
| Nanodrop Spectrophotometer | NN1-Hallway | Greg Brubaker Sandeep Eswarappa |
Nanodrop | 43723 41229 |
| NN Cold Rooms | NN1-Hallway | Pat DiBello Ashish Bhattacharjee |
42916 45508 |
|
| UV-Vis Spectrophotometer DU 640 | NN1-27A | Pat DiBello Ashish Bhattacharjee |
Beckman | 42916 45508 |
| NB Common Equipment | ||||
| L90K Ultracentrifuge | NB2-109 | Diane Greene Lahoucine Izem |
Beckman | 49173 49173 |
| Microplate Luminometer | NB2-37 | Lisa Dechert Smarajit Bandyopadhyay |
Dynex Technologies | 45855 45855 |
| Shimadzu Spectrophotometer UV-1610 | NB2-107 | Paroma Ghosh | Shimadzu | 41229 |
| NE Common Equipment | ||||
| NE Dark Room | NE1-Hallway | Mark Calabro Xin-Min Li |
41029 56466 |
|
| Avanti J-20XPI Centrifuge | NE1-245 | Mike Berk Joseph Lupica |
Beckman | 45932 56466 |
| Avanti J-20XPI Centrifuge | NE1-245 | Mike Berk Calivarathan Latchoumycandane |
Beckman | 45932 41029 |
| Optima L-100 XP Ultracentrifuge | NE1-Hallway | Mike Berk Tom Tallant |
Beckman | 45932 56466 |
| LS-6500 Counter | NE1-241 | Mark Calabro Bob Koeth |
Beckman | 41029 56466 |
| DU800-Spectrophotometer | NE1-244 | Mark Calabro Bruce Levison |
Beckman | 41029 59578 |
| ALLEGRAX12R-Centrifuge (Table Top) | NE1-207 | Mike Berk Zhiping Wu |
Beckman | 45932 56466 |
| ALLEGRAX15R-Centrifuge (Table Top) | NE1-224 | Mike Berk Maryam Zamanian-Daryoush |
Beckman | 45932 51900 |
| ALLEGRAX15R-Centrifuge (Table Top) | NE1-247 | Mike Berk Mahesh Shivananjappa |
Beckman | 45932 41029 |
| Agilent 1100 HPLC | NE1-244 | Mark Calabro Bruce Levison |
Agilent | 41029 59578 |
| Agilent 5973 GCMS | NE1-244 | Mark Calabro Bruce Levison |
Agilent | 41029 59578 |
The LRI has five levels of technical support. These are Technician, Technologist, Senior Technologist, Lead Technologist and Principal Technologist. The job description and promotion criteria for each position are listed below after a brief description of the promotion process. In addition to the criteria listed below, candidates for promotion must also meet Human Resource's minimal requirements of education and relevant work experience for the new position.
The LRI Tech Promotions Committee considers promotions requests once a year, usually in late fall or early winter. A month before the deadline, the LRI Division Office will send an email to department administrators, asking them to inform the Staff and Techs that it is time to submit promotion requests. Prior to submitting a request, the tech, the P.I., and the administrator need to determine whether the applicant meets the minimal degree and experience requirements of Human Resources for the requested position. To request a promotion, the applicant needs to submit to their department administrator:
The department administrator will submit these documents to the LRI Division Office, who will then ask Human Resources to confirm that the applicant meets their minimal requirements of education and relevant work experience for the new position. Approved requests will then be reviewed by the LRI Tech Promotions Committee. Candidates for Lead Technologist and Principal Technologist will also be scheduled for a 20 to 30 minute informal interview with the committee. The reason for the interview is to provide the applicant with an opportunity to discuss their qualifications, their specific research project and their laboratory responsibilities with the committee.
The decision to promote an applicant is based on merit, not length of service. The LRI Tech Promotions Committee does not deny promotion requests because of budgetary constraints. If a promotion is denied, a letter will be sent to the Tech and the P.I. explaining the reason for the Committee's decision and suggesting whether the applicant could be put forth for promotion in the future.
The LRI Tech Promotion Committee uses the following job descriptions and promotion criteria when considering a request for promotion.
Technicians are entry level personnel who are recent college graduates or have less than 1 year of relevant work experience.
Technologists are involved in basic research and contribute to laboratory administration. They assist scientific staff, often taking responsibility for parts of research projects. They may be adept at a range of particular techniques and provide that expertise to several projects. After a period they would be expected to work without direct supervision but would not be independent. They would be responsible for some aspects of laboratory administration including ordering supplies, keeping up stock solutions, monitoring equipment, etc.
Senior Technologists should be able to work with minimum guidance. They should work independently on a research project, design experiments and have innovative input into projects, as evidenced by authorship on research papers. They may be involved in administering budgets and looking after the purchase and maintenance of equipment and common equipment rooms. They may make administrative decisions when the Principal Investigator is away.
Lead Technologist is the first positions in which the technologist makes independent decisions, either scientific or administrative.
Principal Technologists are self-motivated and capable of carrying out independent research at the level of a postdoctoral fellow. They would have a high degree of innovative input and would be expected to achieve a regular output of refereed research papers. They would contribute to the total research effort of the laboratory, by taking on high-risk projects, supervising other staff, and being involved in the selection of support staff. They would assist the Principal Investigator with the construction and submission of grant proposals.
Lerner Research Institute
Cleveland Clinic, Mail Code NB21
9500 Euclid Avenue
Cleveland, Ohio 44195